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Calculate the concentration of αFP in the sample by plotting standard curve
FGL1 present in the sample is added and binds to antibodies coated on the wells
it binds with Ckm bound to precoated antibody
Calculate the concentration of PREX1 in the sample by plotting standard curve
Substrate solution is then added and color develops in proportion to the amount of Human NPR2
Rat IL-12 (Interleukin 12) ELISA Kit Packaging:96 wells Calculate the concentration of αFPThe Rat IL 12 (Interleukin 12) ELISA Kit measures IL 12 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 12 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.